antibody to ran  (Cell Signaling Technology Inc)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Knockdown of glucose-regulated protein 78 decreases the invasion, metalloproteinase expression and ECM degradation in hepatocellular carcinoma cells

doi: 10.1186/1756-9966-31-39

Figure Lengend Snippet: GRP78 knockdown decreased JNK and ERK signaling pathway. (A) Western blot analysis of JNK and p-JNK levels in cells that stably expressing shGRP78-3. (B) Western blot analysis of the ERK and p-ERK levels in cells that stably expressing shGRP78-3. (C) Western blot analysis of FAK, pY397-FAK, Src and pY416-Src in the cells that stably expressing shGRP78-3. All the experiments were repeated for three times, the results of quantative analysis were represented as x ¯ ± SE and analyzed by one-way ANOVA. (Columns,mean of three separate experiments; bars, SE; *, values significantly different at the 5% levels).

Article Snippet: The primary antibodies used were: GRP78 (sc-1051), GRP94 (sc-1794), MMP-2 (CST-4022), MMP-9 (CST-3852), MMP-14 (ab3644), TIMP-1 (CST-8946), TIMP-2 (sc-21735), FAK (396500, Biosource), FAK-pY397 (44625 G, Biosource), JNK (sc-7345), Src(CST-2123), Src-pY416(CST-6943), p-JNK (sc-6354), c-Jun (CST-9165), p-c-Jun (CST-9261).

Techniques: Western Blot, Stable Transfection, Expressing